This protection may be associated with kefir bioactive components including peptides, polysaccharides and sphingolipids. Raw milk has traditionally been used to make kefir. Rate of tumor development, cytokines in serum; mammary gland tissue, and tumor isolated cells were monitored. These include vitamins, fatty acids, and polyphenol antioxidants. already built in. Kefir: a multifaceted fermented dairy product. Dadashi S., Boddohi S., Soleimani N. Preparation, characterization, and antibacterial effect of doxycycline loaded kefiran nanofibers. Foods that may help lower your risk of breast cancer include numerous veggies and fruits, fatty fish, fermented foods, beans, many herbs and spices, whole grains, and walnuts. SDS-PAGE of kefir microorganism. Kefir should be avoided duringradiation treatmentbecause it has been shown to protect cells against cell death caused by radiation damage, raising the possibility that it will lessen the cytotoxic impact of radiation on breast cancer cells. Epub 2009 May 12. The antiproliferative effects of extracts of kefir, yogurt, and pasteurized cow's milk on (1) human breast cancer cells (MCF-7); and (2) normal human mammary epithelial cells (HMECs) was investigated at various doses. The present study results showed that kefir significantly reduced the growth rate of the U87 cell line. At 24-hour test, cell viability in 9mg/ml concentration was 69%, and in 4mg/ml, concentration was 83%. Breast Cancer; IBD ; Migraine; Multiple Sclerosis (MS) Rheumatoid Arthritis; Type 2 Diabetes; Sponsored Topics; Articles. Copyright 2022 Haymarket Media, Inc. All Rights Reserved Accessibility The cells were trypsinized and collected from the flasks and by using hemocytometer slide cell counting was performed. Your use of this website constitutes acceptance of Haymarket Medias Privacy Policy and Terms & Conditions. A. Immuno-modulatory and anti-tumor effects of cuminum cyminum essential oil. Furthermore, studies by de Moreno de LeBlanc et al 5,17 on the administration of commercial milk kefir from Canada in a breast cancer murine model determined that it can slow down cancer by elevating local immune response and selected cytokines. Conditional logistic regression examined breast cancer risk associated with energy-adjusted exposure categories of fat-food variables. In recent years, reports have shown that dietary probiotics such as kefir have a great potential for cancer prevention and treatment. Incident breast cancer cases (n = 237) were matched to controls (n = 673) on age and screening date. 2021 Oct 27;2021:9081738. doi: 10.1155/2021/9081738. The antiproliferative effects of extracts of kefir, yogurt, and pasteurized cow's milk on (1) human breast cancer cells (MCF-7); and (2) normal human mammary epithelial cells (HMECs) was investigated at various doses. Some in-human studies have also been conducted to determine if kefir can improve or prevent chemotherapy-induced toxicities or discomfort. The rate of cell growth assessment demonstrates that in treatment with sample A drink (drink supernatant with 24 hours fermenting duration), by increasing the concentration, cell viability decreases. Numerous investigation have been accomplished in the field of the therapeutic property of probiotic products. Breast cancer-related effects of consuming kefir Four days post-injection, they received kefir or KF on a cyclical basis. The highest quartiles of vegetable oil-based dietary fats (odds ratio, OR = 1.74; confidence interval, CI = 1.12-2.72) and dried soup powders (OR = 1.59; CI = 1.04-2.43) showed positive associations. Multiple in vitro and animal studies suggest that fermented dairy may have anticancer properties. The major flaxseed lignan are glycosides of secoisolariciresinol, however flaxseed also contains small amounts of matairesinol, isolariciresinol, and pinoresinol. Kefir extraction causes the decrease of TGF- gene expression in HuT-102 line of T-lymphocyte cancer cells (HTLV-1-positive malignant T-lymphocyte) and consequently decreases 98% of cell proliferation in treatment with 60mg/ml during 24 hours [19]. This process was also repeated after 48 hours. The MTT test finding demonstrated the effect of the treatment on growth and mortality of the cells by turning the yellow color of tetrazolium solution to purple which is in the result of formazan crystal formation by mitochondrial succinate dehydrogenase enzyme activity. This study demonstrated the modulatory capacity of KF on the immune response in mammary glands and tumors and the importance of the administration period to obtain this effect. In both of the studies at 24 and 48 hours, the most and the least cell viability was observed, respectively, in 1.5 and 20 concentrations. Healthline Media does not provide medical advice, diagnosis, or treatment. One such agent is Kefir. The novel lignan demethoxy-secoisolariciresinol also was tentatively identified in flaxseed. Leafy green vegetables contain. Please login or register first to view this content. In addition, extraction of microorganisms in homogenized kefir and its sedimentation of cell lysate was surveyed, and the findings demonstrated that the produced cell lysate which had a significant viscosity and jelly-like appearance, in applied concentrations, showed a little toxicity. Nutrients. Two of the most common beneficial yeasts in kefir are Saccharomyces cerevisiae and. Eighty percent of primary malignant brain tumors and 30% of all kind of CNS tumors are caused by glioma [3]. The current study aims at examining the effect of kefir on cell survival, proliferation, and motility of colorectal cancer (CRC) cells. Milk fermented by Lactobacillus casei CRL431 administered as an immune adjuvant in models of breast cancer and metastasis under chemotherapy. The results of this systematic review suggest that kefir may be associated with cancer prevention and it also has beneficial effects in cancer treatment. They say the same thing about antioxidant supplements, but they do not really differentiate between healthy cells and cancer cells. Previous studies demonstrated that patients with breast cancer who took kefir extracts experienced a dose-dependent . de Moreno de LeBlanc A, Matar C, Farnworth E, Perdigon G. Cytokine. Balancing your omega-3 to omega-6 ratio by eating more fatty fish and less refined oils and ultra-processed foods may help reduce your breast cancer risk as well (32). After incubation, the culture medium was offloaded, about 200l DMSO solution was added to each well, and then after incubation at room temperature, the optical density (OD) was read at 570nm wavelength by Absorbance Reader (ELx800, BioTek, USA) [11]. Additionally, in a study involving 1,260 Nigerian women, those with the highest intake of beans had up to a 28% reduced risk of breast cancer compared with those with the lowest intake (37). Even with a nutrient-rich diet, you still need regular breast cancer screenings like mammograms and manual checks. Fat From Different Foods Show Diverging Relations With Breast Cancer Risk in Postmenopausal WomenNutrition and Cancer, January 2005 Acid Reflux; ADHD; Allergies; Alzheimer's & Dementia; Bipolar Disorder; Cancer; Crohn's Disease; At 48 studies, this process was observed except for 1.5 and 5mg/ml concentrations. After passaging 3 times, cell count was appropriate for the test. For breaking the cell wall, kefir fungi soaking in PBS was frozen and defreezed at -80C for 10 times. The functionality is limited to basic scrolling. Therefore, a more accurate survey of kefir function mechanism and its effectiveness, inducing apoptosis, are considered in future investigations. Epub 2012 Apr 21. Kefir is recommended for breast cancer Would you like email updates of new search results? All the same, research suggests that these foods may lower your risk of the disease. Water kefir boasts multiple health benefits, like the kind you cant ignore: it kills candida, boosts immunity, fights cancer, supports detox, builds bone density, heals IBS and IBD, and the list goes on. Evaluation of nitric oxide production and proliferation activity of recombinant Bacterioferritin of. It occurs when abnormal cells in your body grow uncontrollably, such as in a tumor. Their omega-3 fats, selenium, and antioxidants like astaxanthin may offer protective effects against cancer (27, 28, 29). This study brought out the potential of the probiotic beverage kefir water in cancer treatment. Use of kefir-derived lactic acid bacteria for the preparation of a fermented soy drink with increased estrogenic activity. and transmitted securely. Studies analyzing data from hundreds of thousands of women have also linked apple and pear intake to a lower risk of breast cancer (23, 24). You may notice problems with Siegel R. L., Miller K. D., Jemal A. But where this functional food really shines is in the area of cancer, with studies having shown that kefir extracts are capable of targeting and destroying malignant cancer cells while protecting healthy cells. Ask a healthcare professional for advice about breast cancer screenings. Int J Food Microbiol. Further analyses demonstrated that kefir-mediated milk fermentation resulted in an increase in peptide concentrations and a change in peptide profiles compared to yogurt or milk. Probiotics aren't the only healthy thing we get from kefir. 1. Scientists also discovered that kefir has an ability to inhibit the proliferation of apoptosis in tumor cells, thanks to peptides and polysaccharides. Kefir is one of the most probiotic foods you can eat. Because of how rich water kefir is in probiotics, it could potentially help to boost immune functions which will ultimately aid the prevention of cancer. Phytoestrogens of the lignan type are widely distributed in plant food items; the richest known dietary source of lignans is flaxseed. Fermented dairy intake has been evaluated as a potential preventive for cancer. The https:// ensures that you are connecting to the Likewise, a large 2015 study linked higher blood levels of total carotenoids to an 18%28% reduced risk of breast cancer as well as a reduced risk of recurrence and death in those who already had breast cancer. At the end of the feeding period, mice were injected subcutaneously with tumor cells in the mammary gland. At a 48-hour period, we observed the same finding as described above (the most cell viability: 90% in 1.5mg/ml concentration and 44% in 20mg/ml concentration). The kefir starter cultures themselves and metabolites of the microorganisms formed during fermentation of kefir lead not only to the formation of lactic acid, acetic acid, ethanol, and carbon dioxide but also to the release of new milk-associated antioxidant micronutrients such as organic acids, amino acids, vitamins (E, B3, B6, and B12), minerals (Se, Fe, Zu, and Mn), and enzymes (glutathione peroxidase, catalase, and superoxide dismutase) [7, 8]. After that, the supernatant was separated from the sediment with centrifuging (20min, 6000rpm). Estrogen receptor beta exerts growth-inhibitory effects on human mammary epithelial cells. Kefir is likewise a foodstuff including a collection of probiotic microorganisms that the majority of these probiotics are lactic acid bacteria (LAB). Kefir extracts suppress in vitro proliferation of estrogen-dependent human breast cancer cells but not normal mammary epithelial cells, Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice, Identification and stereochemical characterization of lignans in flaxseed and pumpkin seeds, Study of cytokines involved in the prevention of a murine experimental breast cancer by kefir, Fat From Different Foods Show Diverging Relations With Breast Cancer Risk in Postmenopausal Women. Food Science and Human Wellness, 2 (3) . The cell cytotoxicity of kefir and sedimentation of cell lysate and extract of kefir was assessed using the MTT test after 24 and 48 hours. Kefir also has varying degrees of benefit for different types of cancer. The Many Faces of Kefir Fermented Dairy Products: Quality Characteristics, Flavour Chemistry, Nutritional Value, Health Benefits, and Safety. Glioblastoma multiforme, which is the most prevalent and the most acute kind of malignant brain tumors in adults (WHO grade IV), is one of the astrocytic glial cells tumors with histology symptoms such as the increase of mitosis, polymorphisms, endothelial proliferation, and necrosis and disseminates to adjacent tissues rapidly [4]. Due to the properties of this drink, kefir can be of interest to the food industry, especially the dairy, for the production of this valuable product. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. According to a segment of test-tube and animal studies, consuming high amounts of isoflavones, a compound found in soy, could increase the risk of breast cancer development. Research is mixed. A 2015 literature review of 27 studies linked consumption of dairy products, including fermented dairy products like yogurt and kefir, to a reduced risk of breast cancer in both Western and Asian populations (34). A study involving 4,706 women found that high bean intake reduced breast cancer risk by up to 20% compared with low bean intake (36). Kefir grains have not been used as a therapeutic choice until now; consequently, the aim of this study is utilizing kefir grains and determining its microorganism's effect on glioma cancer cells. Effect of milk fermented with a Lactobacillus helveticus R389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in miceImmunology & Medical Microbiology, May 2006 In this experimental study, we used a U87 cancer cell line (glioblastoma). 2014 Dec;6(3-4):123-35. doi: 10.1007/s12602-014-9168-0. Previous studies using a mouse model have demonstrated that Lactobacillus helveticus and Lactobacillus casei inhibit the development of fibrosarcoma and colon cancer, respectively. Kefir has also been shown to help in the treatment of high blood pressure, high cholesterol, constipation and diabetes. Download Citation | On Dec 1, 2022, Giacomo Mantegazza and others published Use of kefir-derived lactic acid bacteria for the preparation of a fermented soy drink with increased estrogenic . Moreover, at the 2.5% dose, kefir extracts decreased the MCF-7 cell numbers by 56%, while yogurt extracts decreased MCF-7 cell proliferation by only 14%. Specifically, their high fiber content may protect against breast cancer. Close more info about Fermented Dairy and Cancer. Panowie powinni nauczy si dokonywa waciwych ywieniowych wyborw: chude misa duszone, gotowane, bez panierki, ryby i wdliny, np. Featured. Likewise, the comparison of two treatments at 24-hour period demonstrates that all the concentrations except 1.5mg/ml demonstrated that the sample of kefir drink which was produced during 24 hours fermenting kefir drink supernatant (yielded after 24 hours fermentation) has more cytotoxicity effect in comparison with kefir drink supernatant (yielded after 48 hours fermentation). 2015 Dec;18(12):852-7 Authors: Rafie N, Golpour Hamedani S, Ghiasvand R, Miraghajani M Abstract Some studies have suggested chemopreventive effects of kefir, a fermented milk product, on carcinogenesis. Scientists concluded that "Kefir water inhibited tumour proliferation in vitro and in vivo mainly through cancer cell apoptosis, immunomodulation by stimulating T helper cells and cytotoxic T cells, and anti-inflammatory, antimetastatic, and antiangiogenesis effects." 3.Kefir's anti-inflammatory properties. Kefir is a fermented milk drink that is highly nutritious and contains live probiotics. Peptide content and capillary electrophoresis analyses showed that kefir-mediated milk fermentation led to an increase in peptide concentrations and a change in peptide profiles relative to milk or yogurt. Kefir drink is one of the most important probiotic products, which is made using kefir microorganisms in fermenting the milk. 3 In the review, all of the studies demonstrated some. This study followed 32,826 women over a 20-year period (10). Clipboard, Search History, and several other advanced features are temporarily unavailable. For example, many moisturizers, cosmetics, and hair products contain parabens, a type of chemical that could play a role in the development of breast cancer. No antiproliferative effects of kefir extracts were observed in the HMECs, while the yogurt extracts exerted antiproliferative effects on HMECs at the 5% and 10% doses. All rights reserved. Other studies have similarly shown that kefir induces apoptosis of acute erythroleukemia, myelogenous leukemia, colon cancer, and breast cancer cell lines.2,4-6 One study demonstrated that kefir decreased proliferation of colon cancer cells by arresting the cell cycle at the G1 phase, and, along with other studies, showed that kefir induces apoptosis through a caspase-dependent pathway.2,7, Continuing Medical Education (CME/CE) Courses. Sauerkraut is an incredible food. Some studies have suggested chemopreventive effects of kefir, a fermented milk product, on carcinogenesis. Then, 200cc of fresh skim milk kept at 25C was added to 20gr of kefir fungi and incubated at room temperature in two periods of 24 and 48 hours. KG-1 blood cancer (acute erythroleukemia) cell line treatment with kefir extraction without cell demonstrated the induction of apoptosis and necrosis and also decrease of the erythroleukemia cancer cell line proliferation [17]. It can be hard but necessary for independent people to accept help after a breast cancer diagnosis. Soleimani N., Daneshmandi S., Sattari M., Pourfathollah A. Consumption of soybean, soy foods, soy isoflavones and breast cancer incidence: Differences between Chinese women and women in Western countries and possible mechanisms. The organisms have been shown to inhibit both salmonella and E. Coli in the laboratory. In the present study, additional flaxseed and pumpkin seed lignan glycosides are identified. In the large 2013 study mentioned above, women who consumed at least 2 servings of peaches each week had up to a 41% reduced risk of developing estrogen receptor-negative breast cancer (21). Numerous investigation have been accomplished in the field of the therapeutic property of probiotic products. Kefir, a fermented milk product, exhibits antitumoral activity in vivo; yet its mechanism of action remains elusive. In the result of treatment with kefir fungi sedimentation of cell lysate, at 24-hour test, the most viability rate and the least viability rate were observed in 0.65mg/ml and 7mg/ml concentrations, respectively (IC50). The rate development of tumor cells, IgA (+) cells, and CD4+ and CD8+ T lymphocytes was monitored in mammary . The highest quartiles of vegetable oil-based dietary fats (odds ratio, OR = 1.74; confidence interval, CI = 1.12-2.72) and dried soup powders (OR = 1.59; CI = 1.04-2.43) showed positive associations. Disclaimer, National Library of Medicine Many in vitro and animal studies have evaluated the potential anticancer properties of kefir. Kefir extracts suppress in vitro proliferation of estrogen-dependent human breast cancer cells but not normal mammary epithelial cellsJournal of Medicinal Food, September 2007 Subsequently, purple color is observed when the cell is alive, and by comparison of the color intensity in treated wells and control wells, increase or decrease of cell count will be revealed. Consequently, kefir can be an option for a glioblastoma brain tumor which is the most acute type of glioma, and the rate of the suffering of patients is still low. This issue demonstrates that some of the microbial metabolites in this process can lead to the death of glioblastoma cancer cells line. Fermented foods like yogurt, kimchi, miso, and sauerkraut contain probiotics and other nutrients that may safeguard against breast cancer (33). Moreover, at a 2.5% dose, kefir extracts decreased the MCF-7 breast cancer cell numbers by 56%, while yogurt extracts decreased the breast cancer cells by only 14%. Lactobacillus acidophilus and L. delbrueckii bacterial supernatants revealed their anticancer property on AGS MCS-7 SW620 and HT-29 cancer cell lines by the induction of the caspase-3-dependent apoptosis pathway and reduction of Bcl2 expression. The secretion of TNF- and cytokines such as IL-5 [ 34 ], IL-6 [ 35 ], IL-1 [ 35, 36 ], IL-12 [ 36] is increased, and secretion of IL-8 is decreased. The obtained results of the supernatant and the sedimentation of cell lysate assessment showed that the most rate of the cell death after treatment with bacterial supernatant at 24 hours in 0.024mg/ml concentration and at 48 hours 0.045mg/ml concentration showed 19% and 17% growth reduction, respectively. The researchers found that consuming 2 ounces (57 grams) of walnuts each day for 23 weeks led to significant changes in levels of specific genes that control the growth and spread of breast cancer cells (48). Generating an ePub file may take a long time, please be patient. Here's how a successful editor learned to rely on. official website and that any information you provide is encrypted Register now at no charge to access unlimited clinical news, full-length features, case studies, conference coverage, and more. Bioactive Compounds from Kefir and Their Potential Benefits on Health: A Systematic Review and Meta-Analysis. Jiang L., Zhou J., Zhong D., et al. Some test-tube studies also show that certain compounds found in grapes including flavonoids and anthocyanins can protect against breast cancer cells. Introduction In addition, one 2016 test-tube study showed that certain compounds isolated from walnuts were able to block the growth of breast cancer cells by 63% (49). It has the ability to protect against DNA damage that leads to cancer as shown in multiple studies below. COLON CANCER: Cruciferous vegetables, including cauliflower, cabbage, and broccoli, may help lower your risk of breast cancer (12). Here, the immune cell populations in mammary glands affected by the cyclic consumption of kefir or KF for 2 or 7 d were evaluated using a breast tumor model. Breast Cancer Res Treat. Treeck O, Lattrich C, Springwald A, Ortmann O. Walnuts have a long list of benefits and are a great source of heart-healthy fats, including alpha-linolenic acid (46). The numbers in the parentheses (1, 2, 3) are clickable links to peer-reviewed scientific papers. Arch Iran Med. This probiotic drink has attracted researchers' attention as an option for the treatment of numerous diseases in recent years. An older 2012 analysis of 8 studies in 7,011 women found that those with higher levels of carotenoids had a significantly reduced risk of breast cancer compared with women with lower levels (9). I agree. The aim of this review study was to evaluate the scientific evidence for effects of k. Sonication for 25min was performed, and then kefir whole-cell lysate and sedimentation of cell lysate were denatured in sample buffer for SDS-PAGE as shown in (Figure 1). Extracts of kefir extracts had no observable antiproliferative effects in the normal HMECs, while the yogurt extracts had antiproliferative effects on HMECs at the 5% and 10% doses. To lower your risk of breast cancer, steer clear of alcohol, fast food, fried foods, processed meats, added sugar, and refined carbs. Notably, studies associate other eating patterns like the Mediterranean diet with a reduced risk of breast cancer. Odrobina czerwonego wytrawnego wina, wypijanego okazjonalnie nie zaszkodzi. Mechanisms through which kefir induces anticancer effects Full size image 1. Conclusions: Kefir water inhibited tumor proliferation in vitro and in vivo mainly through cancer cell apoptosis, immunomodulation by stimulating T helper cells and cytotoxic T cells, and anti-inflammatory, antimetastatic, and antiangiogenesis effects. Furthermore, some research suggests that certain skin care products may increase breast cancer risk. Go to: 2. A 2017 test-tube study found that these antioxidants exhibit significant anticancer effects against aggressive breast cancer cell lines (38). Unfermented cow milk extracts increased proliferation of MCF-7 cells and HMECs at concentrations above 0.31%. Female BALB/c mice were injected with American Type Culture Collection 4T1 tumoral cells in the left mammary gland after having been fed for two, five or seven consecutive days with fermented milk. eCollection 2020. To view unlimited content, log in or register for free. Two-day cyclical administration of KF increased IL-10(+) cells in mammary glands and in tumors and decreased IL-6(+) cells in tumor. Two-day cyclical administration of both products delayed tumor growth. Several epidemiologic studies have investigated the association between fermented dairy intake and cancer, although results have been mixed. This article contains scientific references. The .gov means its official. Secoisolariciresinol (but none of the other lignans) has been identified in pumpkin seeds. Recent studies have focused on the mechanism of action of kefir on cancer cells in vitro. It's a fantastic source of other nutrients as well. Interestingly, the study also found that high consumption of cooked onion was associated with an increased risk of breast cancer (15). Federal government websites often end in .gov or .mil. Some of the cancer cells whose growth may be hindered by water kefir include colon cancer, blood cancer, and even breast cancer. Anti-tumorigenic effects have been demonstrated in animal studies from the intake of kefir, a traditional fermented milk product believed to originate from the Caucasian mountains of Russia. These foods changed my life and I have watched them change thousands of other people too. The administration of L. helveticus delayed the development of mammary tumor in every cases, a two- or seven-day feeding period being the most effective. A modified diet history method, a structured questionnaire, and anthropometric measurements provided data. http://foodforbreastcancer.com/foods/kefir. Probiotics Antimicrob Proteins. Previous studies have shown that compounds released during milk fermentation by Lactobacillus helveticus are implicated in the antitumor effect of this product. Effect of kefir drink supernatant (yielded after 48 hours fermentation) on U87 cell viability after 24 and 48 hours (P value 0.5). In the present study, we assessed the cytotoxic effect of kefir on the rate of growth and increase of glioblastoma cancer cell as the most severe form of brain . Kefir is a good source of conjugated linoleic acid (CLA), which has been shown to inhibit the growth and migration of breast cancer cells. According to the above-mentioned explanations, we suppose that kefir microorganisms can be efficacious in prevention and treatment of different cancers. Some of the kefir properties, such as antimicrobial use wound healing, digestive system immunity augment, regulation of cholesterol absorption, and antitumor and antimetastatic effects on various cancer cell lines, are just some of the therapeutic properties of this probiotic foodstuff [16]. In the present study, the antiproliferative effects of extracts of kefir, yogurt, and pasteurized cow's milk on human mammary cancer cells (MCF-7) and normal human mammary epithelial cells (HMECs) was investigated at doses of 0.31%, 0.63%, 1.25%, 2.5%, 5%, and 10% (vol/vol). Researchers found that those with the highest intake of seafood sources of omega-3s had up to a 14% reduced risk of breast cancer compared with those who ate the lowest amount (30). For example, oregano boasts the antioxidants carvacrol and rosmarinic acid. Rate of tumor development, cytokines in serum; mammary gland tissue, and tumor isolated cells were monitored. U87 glioblastoma cancer cell line (supplied from Cell Bank, Tarbiat Modares University, Tehran) was incubated in DMEM-F12 culture medium with 10% PBS, 100 units penicillin-streptomycin antibiotics at 37C and 5% CO2 [12, 13]. Research on soy is mixed, but human studies suggest it may protect against breast cancer. Bioactive peptides in kefir induce activation of macrophages and phagocytosis and nitric oxide (NO) production [ 33 ]. Furthermore, other research suggests that adding whole grains to your diet could also protect against several other types of cancer as well, including pancreatic, colorectal, stomach, and esophageal cancers (45). Then, sonication was implemented 25 times for 25sec and rest for 1min. Products produced during the fermentation process, such as bioactive peptides and compounds, which are reported to have healthful properties and may provide antihypertensive, antimicrobial, and immune-modulatory benefits. In fact, one 2016 study found that consuming at least seven servings of whole grains each week was linked to a significantly lower risk of the development of breast cancer in women (43). Subsequently, 20l of MTT solution was added to each well and incubated at 37C for an hour to form the formazan crystals. Kefir traditional drink is one of the current probiotic drinks in the dairy industry in the world. Rate of tumor development, histological changes, apoptosis, phagocytic index, peritoneal macrophages, -glucuronidase enzyme in peritoneal macrophages, -interferon (INF) and tumor necrosis factor- (TNF-) in blood serum, CD4+, CD8+, interleukin-6 (IL-6), IL-10, TNF- and INF by immunoperoxidase, and -glucuronidase activity in intestinal fluid all were monitored for two months. Soleimani N., Mohabati Mobarez A., Tavakoli-Yaraki M., Farhangi B. Thus, opting for natural skin care, gardening, and cleaning products may decrease your breast cancer risk. A Swedish study of the relationship between various fats in the diet and risk of breast cancer for women aged at least 50 found that fat from fermented milk products was negatively associated with breast cancer risk. Apoptosis was also assayed as another mechanism involved in tumor growth delay. Bethesda, MD 20894, Web Policies Assessment of growth and mortality of cells was accomplished according to the announced protocol. Tips for Exercising with Breast Cancer, Maintaining a Healthy Diet with Breast Cancer, How I Learned to Accept Help After My Breast Cancer Diagnosis, carotenoids like beta cryptoxanthin and beta carotene, flavonoid antioxidants like quercetin, hesperetin, and naringenin. The most rate of cell death after treatment at the 24-hour period in 0.024mg/ml concentration decreased 19% and during 48 hours in 0.045mg/ml concentration decreased 17%. Parabens are considered endocrine disruptors, which means they may have a negative effect on your hormones (65, 66). 3. Rizk S., Maalouf K., Baydoun E. The antiproliferative effect of kefir cell-free fraction on HuT-102 malignant T lymphocytes. Maher E. A., Furnari F. B., Bachoo R. M., et al. CancerTherapyAdvisor.com is a free online resource that offers oncology healthcare professionals a comprehensive knowledge base of practical oncology information and clinical tools to assist in making the right decisions for their patients. Kefir grains consist of a complex living culture of yeasts and bacteria. The authors report that the effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, which is implicated in estrogen synthesis. The software SPSS, version 22.0 (IBM Corp., NY, USA), was used for statistical analysis. The antioxidants protect the healthy cells during therapy, but not the cancer cells. Samples were heated in boiling water for 5 minutes and separated electrophoretically at a constant voltage of 90V. proteins were determined with Coomassie Brilliant Blue R250. Oxid Med Cell Longev. A P value < 0.05 was considered statistically significant. The authors report that the effect was mediated by increased apoptosis and decreased production of pro-inflammatory cytokines, in particular IL-6, which is implicated in estrogen synthesis. Kefir should be avoided duringradiation treatmentbecause it has been shown to protect cells against cell death caused by radiation damage, raising the possibility that it will lessen the cytotoxic impact of radiation on breast cancer cells. Subsequently, it was incubated at 37 on Nutrient Agar and after 24h was checked for assuring lack of live cell and contamination. Goodenberger M. L., Jenkins R. B. Genetics of adult glioma. Likewise, a study involving 582 Iranian women found that high intake of garlic and leeks may protect against breast cancer. It is prepared by inoculating milk (cow, camel, goat, sheep or even soy milk, almond milk) with kefir grains. The antitumor effects of this drink can have a special place in the diet. Making health-promoting lifestyle choices and avoiding potentially harmful chemicals in personal and household products may lower your breast cancer risk. In the present study, additional flaxseed and pumpkin seed lignan glycosides are identified. Maintaining a moderate body weight may also help reduce your risk (63, 64). The present study was designed to evaluate the effects of the consumption of milk fermented by L. helveticus on a mouse model for mammary carcinoma. Female BALB/c mice were injected with American Type Culture Collection 4T1 tumoral cells in the left mammary gland after having been fed for two, five or seven consecutive days with fermented milk. the display of certain parts of an article in other eReaders. Four days post-injection, they received kefir or KF on a cyclical basis. If your doctor gives you the OK, here's a gentle routine to try. Mndez Utz VE, Prez Visuk D, Perdign G, de Moreno de LeBlanc A. Appl Microbiol Biotechnol. Further analyses demonstrated that kefir-mediated milk fermentation resulted in an increase in peptide concentrations and a change in peptide profiles compared to yogurt or milk. The data used to support the findings of this study are available from the corresponding author upon request. In this review, we have focused on anticancer . Dietary fiber did not influence associations. Kefir can be important in the dairy industry. Both kefir and KF increased IL-10 in serum and decreased IL-6(+) cells (cytokine involved in oestrogen synthesis) in mammary glands. Many factors influence your risk of cancer, including lifestyle and genetics. According to World Health Organization (WHO) classification, glioma is classified to astrocytoma, oligodendroglioma, and mixed gliomas such as oligoastrocytoma and ependymoma [1]. But its protective measures is where is really shines. Garlic, onions, and leeks are all allium vegetables. Jednak przede wszystkim naley pi due iloci wody niegazowanej. Kefir has been shown to have antiproliferative activity against MCF-7 human breast cancer cells and this anticancer effect was observed at much lower concentrations than similar effects ofyogurt. 2. As many other herbs and spices have powerful anticancer effects as well, its a good idea to include a wide variety in your diet, such as thyme, curry spice mixes, and ginger (41). Kefir grains were grown in skim milk at room temperature, and the medium was exchanged daily with new culture medium to maintain grain viability [10]. Cruciferous vegetables contain glucosinolate compounds, which your body can convert into molecules called isothiocyanates. The novel lignan demethoxy-secoisolariciresinol also was tentatively identified in flaxseed. Kefir is fermented milk with Caucasian and Tibet origin, made from the incubation of kefir grains with raw milk or water. In 2016, the rate of new suffering in the United States of America was estimated 23770, and among them, 16050 died [2]. It may be equally important to limit or avoid items like alcohol, highly processed meats, and sugary foods and beverages. Whats more, they may also possess powerful cancer-fighting properties. Several studies have demonstrated that milk fermented with L. helveticus decreased the growth rate of mammary tumors in experimental rats and that this involved decreased production of pro-inflammatory cytokines, including IL-6, which is implicated in estrogen synthesis. Breast cancer is the most common type of cancer, with 20,000 new cases a year in . More research involving humans is needed (25, 26). In fact, research ties citrus fruit to a reduced risk of many cancers, including breast cancer. Fermented milk has been shown in one study to have a protective effect against liver cancer in experimental mice. After 24 hours, kefir fungi were detached, contents were homogenized, and about 5ml drink was gathered by centrifugation. Probiotics and the produced metabolites including organic acids, bacteriocins, and peptides can interfere in metabolite pathways such as proliferation regulation, cell distinction, apoptosis, metastasis, and angiogenesis. We are experimenting with display styles that make it easier to read articles in PMC. Kefir has been shown to have antiproliferative activity against MCF-7 human breast cancer cells and this anticancer effect was observed at much lower concentrations than similar effects ofyogurt. Jenab A, Roghanian R, Ghorbani N, Ghaedi K, Emtiazi G. Int J Nanomedicine. Regularly enjoying berries may help lower your risk of certain cancers, including breast cancer. Bourrie B. C. T., Willing B. P., Cotter P. D. The microbiota and health promoting characteristics of the fermented beverage kefir. Secoisolariciresinol (but none of the other lignans) has been identified in pumpkin seeds. An older 2013 literature review of 6 studies involving 8,393 people linked high citrus intake to a 10% reduction in breast cancer risk (19). Dietary fiber did not influence associations. Probiotics have a substantial role in the treatment and prevention of cancers. Previous studies using a mouse model have demonstrated that Lactobacillus helveticus and Lactobacillus casei inhibit the development of fibrosarcoma and colon cancer, respectively. The result of the MTT test, treatment of the cells with the 48-hour fermented drink, demonstrated the most cell cytotoxicity in comparison with the control group. 23 Conclusions. Our experts continually monitor the health and wellness space, and we update our articles when new information becomes available. Kefir water inhibited tumor proliferation in vitro and in vivo mainly through cancer cell apoptosis, immunomodulation by stimulating T helper cells and cytotoxic T cells, and anti-inflammatory, antimetastatic, and antiangiogenesis effects. It is prepared by inoculating milk (cow, camel, goat, sheep or even soy milk, almond milk) with kefir grains. After all, early detection and diagnosis significantly increase survival rates. 1Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, 2Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, 3Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran. Some studies show that eating fatty fish may specifically reduce your risk of breast cancer. The impressive point was lack of death and the effect of the sample on growth that increased by 12% in 0.012mg/ml concentration (Figure 4). Extracts of kefir extracts had no observable antiproliferative effects in the normal HMECs, while the yogurt extracts had antiproliferative effects on HMECs at the 5% and 10% doses. Nevertheless, an in-depth study is needed on the antitumor and antimetastatic effects of kefir in a . Plates were incubated at 37C and 5% CO2 for two times 24 and 48 hours. The interaction between cancer cells and different concentrations of kefir drink and supernatants at 24 and 48 hours was considered. According to a 2015 study involving 201 people, those who consumed the highest amount of walnuts, peanuts, and almonds each week were 23 times less likely to develop breast cancer than those who didnt consume any nuts (47). Keep in mind that many factors are associated with breast cancer development. Publication types Review Systematic Review MeSH terms A systematic review of 11 studies, including 7 in vitro and 4 animal studies, evaluated the potential chemopreventive effects of kefir. For example, engaging in regular exercise, getting enough rest, and avoiding smoking offer significant protection against breast cancer. The ePub format is best viewed in the iBooks reader. An official website of the United States government. A side effect of drugs, time-consuming, costly, and inefficiency of this treatment method, explains the obligation of seeking new therapeutic approaches and solutions for the prevention of suffering from cancer. Effect of kefir drink supernatant (yielded after 24 hours fermentation) on U87 cell viability after 24 and 48 hours (P value 0.5). Kefir and cancer treatment Kefir has been shown to reduce Adriamycin (doxorubicin)-induced multidrug resistance (which reduces the effectiveness of chemotherapy) in colorectal cancer cells. As well as its high nutritional value and excellent taste, kefir and its constituents exhibit a growing number of health-promoting effects including stimulation of the immune system, antihypocholesterolaemic, antihypertensive, antimutagenic, anticarcinogenic, antimicrobial, and antioxidative properties [9]. Both kefir and KF increased IL-10 in serum and decreased IL-6(+) cells (cytokine involved in oestrogen synthesis) in mammary glands. Youve read {{metering-count}} of {{metering-total}} articles this month. Preparation of Kefir Extract Kefir was supplied from Golestan city. While improving your diet can improve your overall health and reduce your cancer risk in general, its only one piece of the puzzle. 2010 Apr;120(3):557-65. doi: 10.1007/s10549-009-0413-2. This article is based on scientific evidence, written by experts and fact checked by experts. Thus, more research on onions and breast health is needed. Lariciresinol was identified in flaxseed and in pumpkin seeds. At the end of the feeding period, mice were injected subcutaneously with tumor cells in the mammary gland. In the investigation of homogenized kefir, fungi extract supernatant effect on, no changing in the process was observed. Chemotherapy is the most common treatment for breast cancer and its metastasis; however, it affects the patients' quality of life. Effect of lysate of microorganism of kefir on U87 cell viability after 24 and 48 hours (P value 0.5). Sedimentation of cell lysate 2. This doesn't negate the fact that more research is needed to . This product can be utilized as a replacement or a complementary therapy of cancer. In the present study, we assessed the cytotoxic effect of kefir on the rate of growth and increase of glioblastoma cancer cell as the most severe form of brain tumors. A Swedish study of the relationship between various fats in the diet and risk of breast cancer for women aged at least 50 found that fat from fermented milk products was negatively associated with breast cancer risk. Here the effects of the consumption, during 2 or 7 days, of kefir or kefir cell-free fraction (KF) on the systemic and local immune responses in mammary glands and tumors using a murine hormone-dependent breast cancer model were studied. Curcumin, the main active compound in turmeric, has also demonstrated significant anticancer properties, as has apigenin, a flavonoid concentrated in parsley (39, 40). However, many other lifestyle choices may affect your cancer risk too. The results in this study are also in accordance with the findings of [19] who confirmed the inverse relationship between kefir and fermented dairy (containing live probiotics) consumption and the incidence of cancer [21]. Kefir grains have not been used as a therapeutic choice until now; consequently, the aim of this study is utilizing kefir grains and determining its microorganism's effect on glioma cancer cells. Conditional logistic regression examined breast cancer risk associated with energy-adjusted exposure categories of fat-food variables. Kefir is an acidic, effervescent fermented milk that is produced using grains consisting of symbionic lactic acid bacteria and yeast that is a source of vitamins A, K, B1, B2, B5, B12, and C; folic acid; and carotene; as well as several amino acids and multiple minerals.2, A systematic review of 11 studies, including 7 in vitro and 4 animal studies, evaluated the potential chemopreventive effects of kefir.3 In the review, all of the studies demonstrated some anticancer property, primarily antiproliferative or decreased proliferative effects on cancer cells and tumor growth inhibition in animal studies. Kefir grains are a mixture of yeast and bacteria, living in a symbiotic association. U87 cells were treated with two samples of kefir drink which were equal in all of the preparation processes except the fermentation duration so as to consider the effect of fermentation duration in drink function. Unfermented milk extracts stimulated proliferation of MCF-7 cells and HMECs at concentrations above 0.31%. The traditional kefir drink is one of the current probiotic products in the world. Health Conditions. The difference is that kefir contains a wider variety of bacteria, and it also contains beneficial yeasts. Research is mixed overall on whether folate intake has a significant impact, positive or negative, on breast cancer risk. This site needs JavaScript to work properly. Sabokbar N., Khodaiyan F. Total phenolic content and antioxidant activities of pomegranate juice and whey based novel beverage fermented by kefir grains. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed (Bio-Rad) [11], with a 4% stacking gel and a12.5% separating gel. Leafy green vegetables Kale, arugula, spinach, mustard greens, and chard are just a few of the leafy green vegetables that may have anticancer properties. Intestinal Caco-2 and HT-29 cell line treatment with kefir extraction demonstrated time- and dose-dependent inhibition of the cell proliferation [19]. There are diverse microorganisms that can be used for this process and they can have different properties and requirements. Herbs and spices contain plant compounds that may help protect against breast cancer. 2021 Jan;105(1):327-340. doi: 10.1007/s00253-020-11007-x. Rafie N., Hamedani S. G., Ghiasvand R., Miraghajani M. Kefir and cancer: a systematic review of literatures. While certain foods may protect against breast cancer, others may increase your risk. Some research has found that intake of folate, a B vitamin concentrated in leafy green vegetables, may help protect against breast cancer. Theres no doubt that your diet can help prevent chronic diseases, including breast cancer. It also occurs in men, although male breast cancer accounts for less than 1% of all breast cancer cases (1, 2). FOIA The present findings suggest that kefir extracts contain constituents that specifically inhibit the growth of human breast cancer cells, which might eventually be useful in the prevention or treatment of breast cancer. Want to view more content from Cancer Therapy Advisor? The present findings suggest that kefir extracts contain constituents that specifically inhibit the growth of human breast cancer cells, which might eventually be useful in the prevention or treatment of breast cancer. A Systematic Review of Literatures Regarding Kefir and Cancer